Journal of Cardio-Thoracic Medicine

Journal of Cardio-Thoracic Medicine

The Predictive Role of MicroRNA19-b in Myocardial Infarction in Rat

Document Type : Original Article

Authors
Danial Falizkaran Yazdi 1
Reza Sahebi 1
Malihe Aghasizadeh 2, 3
Naghmeh Azadi 3
Susan Darroudi 2, 3
Hamideh Ghazizadeh 3
Maryam Mohammadi Bajgiran 3
Gordon A. Ferns 4
Reza Assaran-Darban 1
Majid Ghayour-Mobarhan 3
1 Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
2 Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
3 International UNESCO Center for Health-Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.
4 Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex BN19PH, UK.
10.22038/jctm.2024.71989.1417
Abstract
Introduction: Cardiovascular disease is one of the leading causes of death worldwide. Acute myocardial infarction (AMI) is the most common and deadly disease of all types of cardiovascular disease. Many biomarkers such as troponin, CK-MB and various microRNAs have been shown to be involved in a variety of diseases, especially cardiovascular disease. Circulating MicroRNAs may be important and early biomarkers for the prognosis of cardiovascular disease. The aim of this study was to investigate the predictive role of miRNA-19b as a potential biomarker for AMI.
Materials and Methods: In this case control method we divided 20 rats in Wistar breed (weight 250-300 g) into two groups of myocardial infarctions and healthy. Standard conditions such as a temperature of 25 ° C, enough water and food were created in the animal room of Mashhad Medical School. 150 mg of isoprenaline was dissolved in 2 ml of injected distilled water and the solution was injected subcutaneously for two days. 24 hours after the last injection, myocardial infarction was confirmed by assessment of (CK-MB and troponin).
Results: Based on the findings, levels of troponin I and also CK-MB were higher in the group of rats induced myocardial infarction compared to healthy rats (P<0.001). In addition, the analyzes showed a significant up-regulation for miR-19b expression in the Treat group (P<0.001).
Conclusions: These findings indicate that circulating miRNAs, especially microRNA 19b, could be excellent candidates as biomarkers for heart disease, especially EMI.
Keywords
MicroRNAs
Cardiovascular disease
Acute myocardial infarction
MicroRNA-19b
  1. Veseli BE, Perrotta P, De Meyer GR, Roth L, Van der Donckt C, Martinet W, et al. Animal models of atherosclerosis. European journal of pharmacology. 2017;816:3-13.
  2. Gofman JW, Lindgren F, Elliott H, Mantz W, Hewitt J, Strisower B, et al. The role of lipids and lipoproteins in atherosclerosis. 1950;111(2877):166-86.
  3. Goretti E, Devaux Y. Which future for circulating microRNAs as biomarkers of acute myocardial infarction? Annals of translational medicine. 2016;4(21).
  4. Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, et al. Circulating microRNAs as stable blood-based markers for cancer detection. Proceedings of the National Academy of Sciences. 2008;105(30):10513-8.
  5. Huntzinger E, Izaurralde EJNRG. Gene silencing by microRNAs: contributions of translational repression and mRNA decay. 2011;12(2):99-110.
  6. Ipsaro JJ, Joshua-Tor LJNs, biology m. From guide to target: molecular insights into eukaryotic RNA-interference machinery. 2015;22(1):20-8.
  7. Dharap A, Pokrzywa C, Murali S, Pandi G, Vemuganti RJPo. MicroRNA miR-324-3p induces promoter-mediated expression of RelA gene. 2013;8(11):e79467.
  8. Forman JJ, Legesse-Miller A, Coller HAJPotNAoS. A search for conserved sequences in coding regions reveals that the let-7 microRNA targets Dicer within its coding sequence. 2008;105(39):14879-84.
  9. Zhang J, Zhou W, Liu Y, Liu T, Li C, Wang LJOL. Oncogenic role of microRNA-532‑5p in human colorectal cancer via targeting of the 5'UTR of RUNX3. 2018;15(5):7215-20.
  10. Liebetrau C, Möllmann H, Dörr O, Szardien S, Troidl C, Willmer M, et al. Release kinetics of circulating muscle-enriched microRNAs in patients undergoing transcoronary ablation of septal hypertrophy. Journal of the American College of Cardiology. 2013;62(11):992-8.
  11. Li M, Hu X, Zhu J, Zhu C, Zhu S, Liu X, et al. Overexpression of miR-19b impairs cardiac development in zebrafish by targeting ctnnb1. 2014;33(6):1988-2002.
  12. Lu Y, Thomson JM, Wong HYF, Hammond SM, Hogan BL. Transgenic over-expression of the microRNA miR-17-92 cluster promotes proliferation and inhibits differentiation of lung epithelial progenitor cells. Developmental biology. 2007;310(2):442-53.
  13. Kanuri SH, Ipe J, Kassab K, Gao H, Liu Y, Skaar TC, et al. Next generation MicroRNA sequencing to identify coronary artery disease patients at risk of recurrent myocardial infarction. 2018;278:232-9.
  14. Mozaffarian D, Benjamin EJ, Go AS, Arnett DK, Blaha MJ, Cushman M, et al. Heart disease and stroke statistics—2015 update: a report from the American Heart Association. 2015;131(4):e29-e322.
  15. Kumar D, Jugdutt BIJJoL, Medicine C. Apoptosis and oxidants in the heart. 2003;142(5):288-97.
  16. Rizzacasa B, Amati F, Romeo F, Novelli G, Mehta JLJJotACoC. Epigenetic modification in coronary atherosclerosis: JACC review topic of the week. 2019;74(10):1352-65.
  17. Weintraub WS, Daniels SR, Burke LE, Franklin BA, Goff Jr DC, Hayman LL, et al. Value of primordial and primary prevention for cardiovascular disease: a policy statement from the American Heart Association. 2011;124(8):967-90.
  18. Martinez SR, Gay MS, Zhang LJDdt. Epigenetic mechanisms in heart development and disease. 2015;20(7):799-811.
  19. Nichols M, Townsend N, Scarborough P, Rayner MJEhj. Cardiovascular disease in Europe 2014: epidemiological update. 2014;35(42):2950-9.
  20. Taylor JJEhj. Third universal definition of myocardial infarction. 2012;33(20):2506-7.
  21. Wang W, Zheng HJFiCM. Myocardial Infarction: The Protective Role of MiRNAs in Myocardium Pathology. 2021;8.
  22. Lv Y-C, Tang Y-Y, Peng J, Zhao G-J, Yang J, Yao F, et al. MicroRNA-19b promotes macrophage cholesterol accumulation and aortic atherosclerosis by targeting ATP-binding cassette transporter A1. 2014;236(1):215-26.
  23. Xue Y, Wei Z, Ding H, Wang Q, Zhou Z, Zheng S, et al. MicroRNA-19b/221/222 induces endothelial cell dysfunction via suppression of PGC-1α in the progression of atherosclerosis. 2015;241(2):671-81.
  24. Goretti E, Wagner DR, Devaux Y. miRNAs as biomarkers of myocardial infarction: a step forward towards personalized medicine? Trends in molecular medicine. 2014;20(12):716-25.
  25. Devaux Y, Mueller M, Haaf P, Goretti E, Twerenbold R, Zangrando J, et al. Diagnostic and prognostic value of circulating micro RNA s in patients with acute chest pain. Journal of internal medicine. 2015;277(2):260-71.
  26. Wang K-J, Zhao X, Liu Y-Z, Zeng Q-T, Mao X-B, Li S-N, et al. Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are promising novel biomarkers for early diagnosis of acute myocardial infarction. Cellular Physiology and Biochemistry. 2016;38(3):1015-29.
  27. Huang W, Liu X, Cao J, Meng F, Li M, Chen B, et al. miR-134 regulates ischemia/reperfusion injury-induced neuronal cell death by regulating CREB signaling. 2015;55(4):821-9.
  28. Chi W, Meng F, Li Y, Li P, Wang G, Cheng H, et al. Impact of microRNA-134 on neural cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by targeting HSPA12B. 2014;1592:22-33.
  29. Li C, Fang Z, Jiang T, Zhang Q, Liu C, Zhang C, et al. Serum microRNAs profile from genome-wide serves as a fingerprint for diagnosis of acute myocardial infarction and angina pectoris. 2013;6(1):1-9.
  30. Zeller T, Keller T, Ojeda F, Reichlin T, Twerenbold R, Tzikas S, et al. Assessment of microRNAs in patients with unstable angina pectoris. 2014;35(31):2106-14.
  31. Liu W, Ling S, Sun W, Liu T, Li Y, Zhong G, et al. Circulating microRNAs correlated with the level of coronary artery calcification in symptomatic patients. 2015;5(1):1-10.
  32. Gupta SK, Foinquinos A, Thum S, Remke J, Zimmer K, Bauters C, et al. Preclinical development of a microRNA-based therapy for elderly patients with myocardial infarction. Journal of the American College of Cardiology. 2016;68(14):1557-71.
  33. Li S, Ren J, Xu N, Zhang J, Geng Q, Cao C, et al. MicroRNA-19b functions as potential anti-thrombotic protector in patients with unstable angina by targeting tissue factor. 2014;75:49-57.
  34. Crea F, Liuzzo GJJotACoC. Pathogenesis of acute coronary syndromes. 2013;61(1):1-11.
Journal of Cardio-Thoracic Medicine
Volume 12, Issue 2
Spring 2024
Pages 1323-1328