The Predictive Role of MicroRNA19-b in Myocardial Infarction in Rat

Document Type : Original Article

Authors

1 Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.

2 Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

3 International UNESCO Center for Health-Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.

4 Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex BN19PH, UK.

Abstract

Introduction: Cardiovascular disease is one of the leading causes of death worldwide. Acute myocardial infarction (AMI) is the most common and deadly disease of all types of cardiovascular disease. Many biomarkers such as troponin, CK-MB and various microRNAs have been shown to be involved in a variety of diseases, especially cardiovascular disease. Circulating MicroRNAs may be important and early biomarkers for the prognosis of cardiovascular disease. The aim of this study was to investigate the predictive role of miRNA-19b as a potential biomarker for AMI.
Materials and Methods: In this case control method we divided 20 rats in Wistar breed (weight 250-300 g) into two groups of myocardial infarctions and healthy. Standard conditions such as a temperature of 25 ° C, enough water and food were created in the animal room of Mashhad Medical School. 150 mg of isoprenaline was dissolved in 2 ml of injected distilled water and the solution was injected subcutaneously for two days. 24 hours after the last injection, myocardial infarction was confirmed by assessment of (CK-MB and troponin).
Results: Based on the findings, levels of troponin I and also CK-MB were higher in the group of rats induced myocardial infarction compared to healthy rats (P<0.001). In addition, the analyzes showed a significant up-regulation for miR-19b expression in the Treat group (P<0.001).
Conclusions: These findings indicate that circulating miRNAs, especially microRNA 19b, could be excellent candidates as biomarkers for heart disease, especially EMI.

Keywords

References

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Journal of Cardio-Thoracic Medicine
Volume 12, Issue 2
June 2024
Pages 1323-1328

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